High Performance
AMINO COLUMNS – Amino Universal Premium & Amino Inject Column
Most efficient method to separate Basic Compounds
Comparison of amino-bonded silica gel (NH2) and silica gel (SiOH)
TLC Image (SiOH)
TLC Image (NH2)
①4-Methylpyridine and
②Lidocaine tail on both silica gel TLC and column, resulting in a poor separation.
【Note】If a sample such as an aldehyde having an α-proton is run on an amino-bonded silica column, a Schiff base between sample and amino-bonded silica gel will be formed, and the sample will never be eluted. Acetone cannot be used either as mobile phase or washing solvent.
Comparison of Amino-bonded silica gel and regular silica gel with added triethylamine as buffer
TLC Image (SiOH)
TLC Image (NH2)
It is not necessary to add buffer (a base) to mobile phase to run a basic compound using Yamazen Amino Column, which does not require time-consuming and cumbersome work, such as preparation of solvent, mobile phase change, flushing the buffer (base) out of the system line, etc. Basic compounds can be separated most efficiently using Yamazen Amino Column.
■Universal Column Premium (NH2)
| Adsorbent | Particle (µm) |
Pore (Å) |
Column Size |
OD x Length (cm) |
Amount (g) |
Packing Size | Catalogue No. |
| Amino (NH2) |
20-40 | 60 | S | 1.8 x 11.4 | 7 | 20 | UW199-02 |
| 50 | UW199-05 | ||||||
| M | 2.3 x 12.3 | 16 | 10 | UW192-01 | |||
| 50 | UW192-05 | ||||||
| L | 3.0 x 16.5 | 40 | 10 | UW193-01 | |||
| 50 | UW193-05 | ||||||
| 2L | 3.0 x 20.0 | 55 | 10 | UW194-01 | |||
| 50 | UW194-05 | ||||||
| 3L | 5.0 x 19.0 | 135 | 10 | UW198-01 | |||
| 4L | 5.2 x 23.0 | 200 | 10 | UW195-01 | |||
| 5L | 6.4 x 24.0 | 300 | 5 | UW196-06 |
■Injection Column (NH2)
| Column Size |
To be used with |
Packing Size | Product No. | Column Size |
To be used with |
Packing Size | Product No. | |
| 3S | Hi-Flash Column Size: S, M |
10 | W899-01 | L | Hi-Flash Column Size: 3L |
10 | W898-01 | |
| 50 | W899-05 | 50 | W898-05 | |||||
| 100 | W899-10 | 100 | W898-10 | |||||
| 2S | 10 | W895-01 | 2L | Hi-Flash Column Size:3L, 4L, 5L |
10 | W885-01 | ||
| 50 | W895-05 | 50 | W885-05 | |||||
| 100 | W895-10 | 100 | W885-10 | |||||
| S | Hi-Flash Column Size:M, L, 2L |
10 | W896-01 | 3L | Hi-Flash Column Size:4L, 5L |
10 | W886-01 | |
| 50 | W896-05 | 50 | W886-05 | |||||
| 100 | W896-10 | 100 | W886-10 | |||||
| M | Hi-Flash Column Size:L, 2L, 3L |
10 | W897-01 | 4L | Hi-Flash Column Size:5L Ultra Pack Size: D, E |
10 | W887-01 | |
| 50 | W897-05 | 50 | W887-05 | |||||
| 100 | W897-10 | 100 | W887-10 | |||||
| L | Hi-Flash Column Size: 3L |
10 | W898-01 | 5L | 10 | W888-01 | ||
| 50 | W898-05 | 50 | W888-05 | |||||
| 100 | W898-10 | 100 | W888-10 |
■TLC (NH2)
| Cat No | Product | Size (cm) |
Thickness of coated adsorbent (mm) |
No. of TLC plates per package |
| 7522 | Aminopropyl Si-(CH2)3NH2 With fluorescence indicator (Amino for flash chromatography NH2 40-63µm) |
2.5 x 7.5 | 0.25 | 10 |
