Simple and User-friendly Software

Parallel and Independent Operation of Each Channel by a group or a single person.
EPCLC W-Prep 2XY

Two chromatograms (Ch-1 & Ch-2) can be viewed on the same display at the same time.

EPCLC W-Prep 2XY

Choose a column, click the [Sample load] button, input TLC Rf values of the target compound and the nearest impurity, and the maximum sample load will be calculated and given automatically. If a different column is selected, the maximum sample load will change accordingly. It will be a great guide for you to decide the proper column for running your sample.

Yamazen is the one and only company that have successfully transferred the method from TLC to column chromatography, based on the true theory of chromatography.



Green Flash Chromatography

     Linear gradient is set with Rf value on TLC. (Rf gradient)

     The optimum method can be automatically developed to elute the target compound at around 4-Column Volume.

     Yellow arrow(s) predicts the eluting position of the target compound(s).


Click to enlarge the image

(Patent No.4087395)

2-Step Gradient is the best method to use when separating two target compounds, of which TLC Rf values (Rf1&Rf2/(3)&(6)) are far apart from each other (ΔRf>0.3) with some other compounds in between. As Fig 1 shows, 2-Step Gradient purifies multiple compounds efficiently while achieving a high resolution. And Rf1 (Compound 3) and Rf2 (Compound 6) elute at 4 to 5 column volume on each gradient.

EPCLC W-Prep 2XY

Compared with the conventional ISOCRATIC method, 2-Step Gradient method shortens run time considerably, saves solvents and energy, and boosts productivity, therefore, It is eco-friendly.

A small amount of sample is run first for a preliminary run, since there is no correlation between TLC and column chromatography. When the preliminary run ends, click the peak of the target compound. The optimum Gradient method for a real run will be automatically developed, like the one for the normal phase chromatography.

(For more information, see Yamazen technical bulletin no.61.)

EPCLC W-Prep 2XY

Benefit of Using an Inject Column for Sample Loading (1)

Inject Column will make sample bands narrower to achieve a higher resolution. Moreover, a larger size sample can be separated by small size column with use of the Inject Column.

EPCLC W-Prep 2XY

Benefit of Using an Inject Column for Sample Loading (2)

Method to Achieve Good Separation of Sample Dissolved in Highly Polar Solvent

No “Coelute”!

Highly polar solvent used to dissolve the sample often causes poor sample separation in flash chromatography. It is, therefore, advisable to use low polar solvent to dissolve the sample. Many times, however, it is necessary to use highly polar solvent when dissolving the tough-to-dissolve sample. Yamazen’s Inject Column eliminates the harmful effect caused by highly polar solvent used to dissolve the sample, by trapping the highly polar solvent. By using Inject column together with main separation column such as Universal column, Hi-Flash column, etc. available from Yamazen, good sample separation can be achieved.

EPCLC W-Prep 2XY

A New Innovative Software to Decide Recommended Size of Inject Column that Eliminates Harmful Effect on Chromatography Caused by Highly Polar Solvent Used to Dissolve Sample (…. will be available soon.)

Input the solvent name and the amount of the solvent used to dissolve a sample, and the recommended size of Inject column that eliminates the harmful effect caused by highly polar solvent used to dissolve the sample, will be decided automatically and pop up in the window.

EPCLC W-Prep 2XY

Detecting capability over a broad range is critically important in flash chromatography!

When running a small size sample or a sample that has low UV absorbance:

Yamazen UV detector is capable of detecting a sample with very high sensitivity. As the chromatograms below shows, these samples are accurately detected and beautifully separated at 0.08 AUFS and 0.04 AUFS on a Yamazen UV detector. Ordinary UV detectors of other makes, however, lack the sensitivity, thus, end up losing precious samples.

EPCLC W-Prep 2XY

Sample: Butyl p-hydroxybenzoate, 0.5mg Methyl p-hydroxybenzoate, 0.5mg Toluene, 9mg
Column used: Yamazen's Hi-Flash, L (30 gram) Fractionation mode: Peak mode separation

EPCLC W-Prep 2XY

When running a large scale sample or a sample that has high UV absorbance:

EPCLC W-Prep 2XY

Sample: Butyl p-hydroxybenzoate, 500mg Methyl p-hydroxybenzoate, 500mg Toluene, 900mg
Column used: Yamazen's Hi-Flash, 2L (45 gram)   Fractionation mode: Peak mode separation

EPCLC W-Prep 2XY

A solvent delivery pump is of high precision medium pressure valveless ceramic piston pump, featuring infinite fine flow adjustments. The gradient at low concentration of solvent as low as 1 to 7% is possible, which is required when using a high polar solvent, like methanol, without pre-mixing.

Effective ceramic piston movement for maximum solvent and bubble clearing, assuring accurate solvent delivery. Bubbles produced by solvents that have low boiling points like dichloromethane will not affect pump.

EPCLC W-Prep 2XY

The precision pressure mater makes it possible to do real time monitoring of the pressure building up inside the column. When pressure hits the preset limit, the pump will stop as quickly as a flash, which will protect the system from being damaged. Highly responding pressure moderation system (PMS) prevents disposable plastic column and glass column from bursting, and allows the sample run to continue.

Safety First

Yamazen's motto is "Safety First!"



Yamazen RI-31 RI detector and ELSD-100X evaporative light-scattering detector are designed for use with” Smart Flash” purification systems to purify organic compounds with little or no chromophores such as Carbohydrates, Steroids, Lipids, Terpenes and other compounds that have no UV absorbance. Parallel detection between UV detector and RI detector or ELSD is possible with Yamazen flash chromatography system.

EPCLC W-Prep 2XY

System Specifications


Pump (with an integrated mixer)
Model No. 580D
Flow Path Two Flow Paths (Independently controlled)
Number of Pump Head Two (Independently controlled)
Flow Rate 0~80ml/min (Independently controlled by PC)
Rated Pressure 1.0 Mpa
Number of solvents mixed Two solvents (Selection from 4 solvents. PC controls each path independently.)
Dimensions 180Wx394Dx317H (mm)
Weight 14Kg
Fixed Wavelength UV Detector
Model No. prepUV-254W
Flow Path Two (Independently controlled)
Light source Hg lamp
Wavelength 254nm
Detector range 0.08-5.12 AUFS
Dimensions 180Wx394Dx160H (mm)
Weight 7Kg
Variable Wavelength UV Detector
Model No. prepUV-10VWH (Single wavelength)
prepUV-20VWH (2-wavelength)
Flow Path Two (Independently controlled)
Light source D2 lamp
Wavelength 200-400nm
Detector range 0.02-10.24 AUFS
Dimensions 180Wx394Dx135H (mm) x 2ea
Weight 4.5kg x 2ea
Variable Wavelength UV Visible Detector
Model No. prepUV/VIS-10VWH (Single wavelength)
prepUV/VIS-20VWH (2-wavelength)
Flow Path Two (Independently controlled)
Light source D2 lamp and halogen lamp
Wavelength 200-800nm
Detector range 0.02-10.24 AUFS
Dimensions 180Wx394Dx135H (mm) x 2ea
Weight 4.5kg x 2ea
Fraction Collector
Model No. FR-260
Detecting Flow Path Two (Independently controlled)
Number of Rack Two (One for each channel)
Driving mode X-Y driven, independently controlled, dual channel
Fraction mode Time Mode, Peak Mode, Peak & Slope mode
(Each rack is controlled independently by PC)
Input Signal Purpose-built signal
Dimensions 320Wx480Dx520H (mm)
Weight 23kg
System Controller (built into Fraction Collector)
Input/Output Signal Pump, Detector, Fraction collector, PC Serial Interface
PC Specifications
OS Windows XP, 7 (32bit)
CPU Pentium III or Mobile Celeron with 500MHz or more or Atom D410 is recommend.
Memory 1GB or more
Monitor Recommended XGA or higher resolution
Hard Disk Space At least 150GB of availability memory
Input/Output Signal Serial RS232C D-sub 9 pin or USB. (for USB, an adaptor is required)
Others Input device (Keyboard, Mouse)

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Standard Accessories

FR-260 Rack
107Wx335Lx159mmH

FR-260 Fraction Collector holds two racks - either two of the same size rack for both channels or one each of the different size rack for each channel. Unless otherwise specified, two WP18150, 18x150mm racks, will be supplied with the system as standard accessory.

Note:
Specifications can change without notice.

Rack Specifications, Collection Tube (O.D. x Length x Quantity)
Cat. No.
Size Code
Fraction Volume(Default)
American Standard Tube Size (mm)
WP13100
A
7ml
13x100x60 pieces
WP15125
A
12ml
15x125x60 pieces
WP16125
A
15ml
16x125x60 pieces
WP16150
A
16ml
16x150x60 pieces
WP18150
B
22ml
18x150x60 pieces
WP25150
C
45ml
25x150x30 pieces
WP25200
C
56ml
25x200x30 pieces
WP20150
D
27ml
20x150x52 pieces
WP30200
E
100ml
*30x200x27 pieces
Note: Test tube with * is of Japanese standard (E-size).

Cat. No.
Size Code
Fraction Volume(Default)
Japanese Standard Tube Size (mm)
WP15150
A
15ml
15x150x60 pieces
WP18180
B
30ml
18x180x60 pieces
WP24180
C
60ml
24x180x30 pieces
WP21180
D
42ml
21x180x52 pieces
WP30180
E
90ml
30x180x27 pieces
WP30200
E
100ml
30x200x27 pieces


Fittings & Tubing

Options
Solvent Level Monitor
Waste Fluid Monitor
External Nozzle Cleaning System
Column Air Purge
TLC Image Reader


download brochure(PDF) EPCLC W-Prep 2XY


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