Yamazenusa

User-friendly Software with Ample Functions and Capabilities

State-Of-The-Art Software Based On True Theory of Chromatography

Yamazen's proprietary automatic method set-up is based on true theory of the chromatography, thus achieves Gradient optimization.

Green Flash Chromatography

Linear gradient is set with Rf value on TLC. (Rf gradient)

The optimum method can be automatically developed to elute the target compound at around 4-Column Volume.

Yellow arrow(s) predicts the eluting position of the target compound(s).

Click to enlarge the image

2-Step Gradient separates multiple compounds efficiently.

2-Step Gradient is the best method to use when separating two target compounds, of which TLC Rf values (Rf1&Rf2/(3)&(6)) are far apart from each other (ΔRf＞0.3) with some other compounds in between. As Fig 1 shows, 2-Step Gradient purifies multiple compounds efficiently while achieving a high resolution. And Rf1 (Compound 3) and Rf2 (Compound 6) elute at 4 to 5 column volume on each gradient.

Compared with the conventional ISOCRATIC method, 2-Step Gradient method shortens run time considerably, saves solvents and energy, and boosts productivity, therefore, It is eco-friendly.

With the use of an Inject (Loading) Column, sample loading can be easily done without any sample loss. No cross contamination.

Yamazen's UV detector monitors chromatogram in a broad OD range.
No Peak Saturation, No Sample Loss.

When running a small size sample and/or a sample that has low UV absorption;

Sample: Butyl p-hydroxybenzoate, 0.5mg Methyl p-hydroxybenzoate, 0.5mg Toluene, 9mg
Column used: Yamazen's Hi-Flash, L (30 gram) Fractionation mode: Peak mode separation

When running a large scale sample and/or a sample that has high UV absorption;

Sample: Butyl p-hydroxybenzoate, 500mg Methyl p-hydroxybenzoate, 500mg Toluene, 900mg
Column used: Yamazen's Hi-Flash, 2L (45 gram)　　　Fractionation mode: Peak mode separation

It's possible to get a slow gradient from 1% to 7% of polar solvent.

A solvent delivery pump is of high precision medium pressure valveless ceramic piston pump, featuring infinite fine flow adjustments. The gradient at low concentration of solvent as low as 1 to 7% is possible, which is required when using a high polar solvent, like methanol, without pre-mixing.

Effective ceramic piston movement for maximum solvent and bubble clearing, assuring accurate solvent delivery. Bubbles produced by solvents that have low boiling points like dichloromethane will not affect pump.

Real Time Pressure Monitor

The precision pressure meter makes it possible to do real time monitoring of the pressure building up inside the columns. Pressure moderation system (PMS) protects disposable plastic column and glass column from bursting.

Yamazen's motto is "Safety First!"

Modular & Open System: easy to maintain and service

System Specifications

Solvent Delivery Pump
Model	No.580S
Flow rate	0-80ml/min.
Rated pressure	1.0 Mpa (145 psi)
No. of solvent mixed	2 (Choose from 4 solvents)
Dimension	140Wx350Dx280mmH
Weight	10 kg

Fixed Wavelength UV Detector
Model	prepUV-254
Light source	Hg lamp
Wavelength	254nm
Range	0.08-5.12 AUFS
Output to recorder	10mVFS
Output to integrator	0-5.12V/AU
Output to D-sub	Purpose built signal
Dimension	140Wx350Dx135mmH
Weight	5 kg

Variable Wavelength UV Detector
Model	prepUV-10V
Light source	D2 lamp
Wavelength	190-360nm
Range	0.08-5.12 AUFS
Output to recorder	10mVFS
Output to integrator	0-5.12V/AU
Output to D-sub	Purpose built signal
Dimension	140Wx350Dx135mmH X 2
Weight	12 kg

UV Visible Detector
Model	prepUV-VIS-10V
Wavelength	190-600nm

Fraction Collector
Model	FR-360
No. of Rack	2 (A pair of the same size rack)
Driving mode	X-Y driven
Fraction mode	Time, Peak, Peak & Slope (Valley), Manual Collect
Input signal	Purpose-built signal
Dimension	320Wx438Dx522mmH
Weight	16 kg

System Controller (built into Fraction Collector)
Input/Output Signal	Pump, Detector, Fraction Collector, PC dedicated

PC Specifications
OS	Windows XP, 7 (32bit)
CPU	Pentium III or Mobile Celeron with 500MHz or more or Atom D410 is recommend.
Memory	1GB or more
Monitor	XGA or higher resolution recommended
Hard Disk Space	At least 150GB of availability memory
Input/Output Signal	Serial RS232C D-sub 9 pin or USB

* For more specifications of the PC, please inquire.

Standard Accessories

A pair of the same size rack

FR-360 Rack
118Wx335Lx159mmH

Note: FR-360 fraction collector holds a pair of the same size racks.
When ordering the rack, please specify it with Cat. Number.

Rack Specifications, Collection Tube (O.D. x Length x Quantity)

Cat. No.	Pin Code	Fraction Volume(Default)	American Standard Tube Size (mm)
AI13100	A	7ml	13x100x75 pieces
AI15125	A	12ml	15x125x75 pieces
AI16125	A	15ml	16x125x75 pieces
AI16150	A	16ml	16x150x75 pieces
AI18150	B	22ml	18x150x75 pieces
AI25150	C	45ml	25x150x30 pieces
AI25200	C	56ml	25x200x30 pieces
AI30200	D	100ml	*30x200x27 pieces
AI60180	E	350ml	*60x180x10 pieces

Note: Test tube with * is of Japanese standard.

Cat. No.	Pin Code	Fraction Volume(Default)	Japanese Standard Tube Size (mm)
AI15150	A	15ml	15x150x75 pieces
AI18180	B	30ml	18x180x75 pieces
AI24180	C	60ml	24x180x75 pieces
AI30180	D	90ml	30x180x27 pieces
AI30200	D	100ml	30x200x27 pieces
AI16180	E	350ml	60x180x10 pieces

Fittings & Tubing

Options
Solvent Level Monitor
Waste Fluid Monitor
Column Air Purge Pump
TLC Image Reader

download brochure(PDF) EPCLC AI-580 Series

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