AI-580S

Click to enlarge the image

Choose a column, click the [Sample load] button, input TLC Rf values of the target compound and the nearest impurity, and the maximum sample load will be calculated and given automatically. If a different column is selected, the maximum sample load will change accordingly. It will be a great guide for you to decide the proper column for running your sample.

Yamazen is the one and only company that have successfully transferred the method from TLC to column chromatography, based on the true theory of chromatography.



Green Flash Chromatography

     Linear gradient is set with Rf value on TLC. (Rf gradient)

     The optimum method can be automatically developed to elute the target compound at around 4-Column Volume.

     The eluting position of the target compound(s) is shown by yellow arrow(s).

Click to enlarge the image

(Patent No.4087395)

2-Step Gradient is the best method to use when separating two target compounds, of which TLC Rf values (Rf1&Rf2/(3)&(6)) are far apart from each other (ΔRf>0.3) with some other compounds in between. As Fig 1 shows, 2-Step Gradient purifies multiple compounds efficiently while achieving a high resolution. And Rf1 (Compound 3) and Rf2 (Compound 6) elute at 4 to 5 column volume on each gradient.

AI-580S

Compared with the conventional ISOCRATIC method, 2-Step Gradient method shortens run time considerably, saves solvents and energy, and boosts productivity, therefore, It is eco-friendly.

A small amount of sample is run first for a preliminary run, since there is no correlation between TLC and column chromatography. When the preliminary run ends, click the peak of the target compound. The optimum Gradient method for a real run will be automatically developed, like the one for the normal phase chromatography.

(For more information, see Yamazen technical bulletin no.61.)

AI-580S

Benefit of Using an Inject Column for Sample Loading (1)

Inject Column will make sample bands narrower to achieve a higher resolution. Moreover, a larger size sample can be separated by small size column with use of the Inject Column.

AI-580S

Benefit of Using an Inject Column for Sample Loading (2)

Method to Achieve Good Separation of Sample Dissolved in Highly Polar Solvent

No “Coelute”!

Highly polar solvent used to dissolve the sample often causes poor sample separation in flash chromatography. It is, therefore, advisable to use low polar solvent to dissolve the sample. Many times, however, it is necessary to use highly polar solvent when dissolving the tough-to-dissolve sample. Yamazen’s Inject Column eliminates the harmful effect caused by highly polar solvent used to dissolve the sample, by trapping the highly polar solvent. By using Inject column together with main separation column such as Universal column, Hi-Flash column, etc. available from Yamazen, good sample separation can be achieved.

AI-580S

A New Innovative Software to Decide Recommended Size of Inject Column that Eliminates Harmful Effect on Chromatography Caused by Highly Polar Solvent Used to Dissolve Sample (…. will be available soon.)

Input the solvent name and the amount of the solvent used to dissolve a sample, and the recommended size of Inject column that eliminates the harmful effect caused by highly polar solvent used to dissolve the sample, will be decided automatically and pop up in the window.

AI-580S

Detecting capability over a broad range is critically important in flash chromatography!

When running a small size sample or a sample that has low UV absorbance:

Yamazen UV detector is capable of detecting a sample with very high sensitivity. As the chromatograms below shows, these samples are accurately detected and beautifully separated at 0.08 AUFS and 0.04 AUFS on a Yamazen UV detector. Ordinary UV detectors of other makes, however, lack the sensitivity, thus, end up losing precious samples.

AI-580S

Sample: Butyl p-hydroxybenzoate, 0.5mg Methyl p-hydroxybenzoate, 0.5mg Toluene, 9mg
Column used: Yamazen's Hi-Flash, L (30 gram) Fractionation mode: Peak mode separation

AI-580S

When running a large scale sample or a sample that has high UV absorbance:

AI-580S

Sample: Butyl p-hydroxybenzoate, 500mg Methyl p-hydroxybenzoate, 500mg Toluene, 900mg
Column used: Yamazen's Hi-Flash, 2L (45 gram)   Fractionation mode: Peak mode separation

AI-580S

A solvent delivery pump is of high precision medium pressure valveless ceramic piston pump, featuring infinite fine flow adjustments. The gradient at low concentration of solvent as low as 1 to 7% is possible, which is required when using a high polar solvent, like methanol, without pre-mixing.

Effective ceramic piston movement for maximum solvent and bubble clearing, assuring accurate solvent delivery. Bubbles produced by solvents that have low boiling points like dichloromethane will not affect pump.

AI-580S

The precision pressure mater makes it possible to do real time monitoring of the pressure building up inside the column. When pressure hits the preset limit, the pump will stop as quickly as a flash, which will protect the system from being damaged. Highly responding pressure moderation system (PMS) prevents disposable plastic column and glass column from bursting, and allows the sample run to continue.

Safety First

Yamazen's motto is "Safety First!"

Yamazen RI-31 RI detector and ELSD-100X evaporative light-scattering detector are designed for use with” Smart Flash” purification systems to purify organic compounds with little or no chromophores such as Carbohydrates, Steroids, Lipids, Terpenes and other compounds that have no UV absorbance. Parallel detection between UV detector and RI detector or ELSD is possible with Yamazen flash chromatography system.

AI-580S



System Specifications


Solvent Delivery Pump
Model No. No.580S
Flow rate 0-80ml/min.
Rated pressure 1.0 Mpa (145 psi)
No. of solvent mixed 2 (Choose from 4 solvents)
Dimensions 140Wx350Dx280H (mm)
Weight 10kg
Fixed Wavelength UV Detector
Model No. prepUV-254
Light source Hg lamp
Wavelength 254nm
Detector range 0.08-5.12 AUFS
Dimensions 140Wx350Dx135H (mm)
Weight 5kg
Variable Wavelength UV Detector
Model No. prepUV-10VH (Single wavelength)
prepUV-20VH (2-wavelength)
Light source D2 lamp
Wavelength 200-400nm
Detector range 0.02-10.24 AUFS
Dimensions 140Wx350Dx185H (mm)
Weight 4.5kg
Variable Wavelength UV Visible Detector
Model No. prepUV/VIS-10VH (Single wavelength)
prepUV/VIS-20VH (2-wavelength)
Light source D2 lamp and halogen lamp
Wavelength 200-800nm
Detector range 0.02-10.24 AUFS
Dimensions 140Wx350Dx185H (mm)
Weight 4.5kg
Fraction Collector
Model No. FR-360
No. of Rack 2 (A pair of the same size rack)
Driving mode X-Y driven
Fraction mode Time, Peak, Peak & Slope (Valley), Manual Collect
Input signal Purpose-built signal
Dimensions 320Wx438Dx522H (mm)
Weight 16kg
System Controller (built into Fraction Collector)
Input/Output Signal Pump, Detector, Fraction Collector, PC dedicated
PC Specifications
OS Windows XP, 7 (32bit)
CPU Pentium III or Mobile Celeron with 500MHz or more or Atom D410 is recommend.
Memory 1GB or more
Monitor XGA or higher resolution recommended
Hard Disk Space At least 150GB of availability memory
Input/Output Signal Serial RS232C D-sub 9 pin or USB
* For more specifications of the PC, please inquire.

Standard Accessories

A pair of the same size rack

FR-360 Rack
118Wx335Lx159mmH

Note: FR-360 fraction collector holds a pair of the same size racks.
When ordering the rack, please specify it with Cat. Number.

Rack Specifications, Collection Tube (O.D. x Length x Quantity)
Cat. No.
Pin Code
Fraction Volume(Default)
American Standard Tube Size (mm)
AI13100
A
7ml
13x100x75 pieces
AI15125
A
12ml
15x125x75 pieces
AI16125
A
15ml
16x125x75 pieces
AI16150
A
16ml
16x150x75 pieces
AI18150
B
22ml
18x150x75 pieces
AI25150
C
45ml
25x150x30 pieces
AI25200
C
56ml
25x200x30 pieces
AI30200
D
100ml
*30x200x27 pieces
AI60180
E
350ml
*60x180x10 pieces
Note: Test tube with * is of Japanese standard.

Cat. No.
Pin Code
Fraction Volume(Default)
Japanese Standard Tube Size (mm)
AI15150
A
15ml
15x150x75 pieces
AI18180
B
30ml
18x180x75 pieces
AI24180
C
60ml
24x180x75 pieces
AI30180
D
90ml
30x180x27 pieces
AI30200
D
100ml
30x200x27 pieces
AI16180
E
350ml
60x180x10 pieces

Fittings & Tubing

Options
Solvent Level Monitor
Waste Fluid Monitor
Column Air Purge Pump
TLC Image Reader



download brochure(PDF) EPCLC AI-580 Series


go to top